Selection of organ-type antigens in normal and diseased tissues

Wolf D. Kuhlmann

Laboratory Diagnostics & Cell Science, 56112 Lahnstein

Thyroid gland: staining of Calcitonin in C cells of thyroid gland. Hemalaun counterstain

Mamma carcinoma metastatic into lymph node: Cytokeratin immunostaining (chromogen: aminoethylcarbazole)

Mamma carcinoma: detection of Cytokeratin in carcinoma cells metastatic into lymph node (chromogen: aminoethylcarbazole). Hemalaun counterstain

Pituitary gland: FSH staining in gonadotrophs (chromogen: diaminobenzidine). Hemalaun counterstain

Human stomach: immunostaining of gastrin in pyloric antrum mucosa cells

Lymphoid cells: HIV p24 antigen staining in infected lymph cells (chromogen: aminoethylcarbazole). Slight hemalaun counterstain

Tonsil: immunoperoxidase staining of immunoglobulins. IgA stained with chromogen aminoethylcarbazole, and IgG stained with chromogen diaminobenzidine (hemalaun counterstain)

Pancreas: tissue sections immunostained for insulin and somatostatin, respectively. Hemalaun counterstain

Lymphatic tissue: muramidase immunostaining of non-lymphocytic cells (chromogen: diaminobenzidine). Hemalaun counterstain

Prostate gland: PSA immunostaining of prostate gland epithelium (chromogen: diaminobenzidine). Hemalaun counterstain

Liver: liver tissue section incubated with serum from patient with autoimmune hepatitis. Antibody binding detected by indirect immunoperoxidase method (chromogen: amioethylcarbazole). Note staining of canaliculi biliferi (syn. bile canaliculi). No counterstain